Summary
Column
Input DNA
Molecule size distribution
| Filter | Molecules | Barcodes | N50_LPM | Weighted_mean | N75 | N50 | N25 | Max |
|---|---|---|---|---|---|---|---|---|
| Reads ≥ 4 | 2096037 | 1048350 | 3.5 | 95841.75 | 60956 | 91777 | 125129 | 546118 |
| Reads ≥ 6 | 1062046 | 698167 | 4.5 | 100626.45 | 66726 | 95181 | 127974 | 546118 |
Long Ranger
Column
GEMs
Number of GEMs     1,142,731
Mean DNA per GEM Â Â Â Â 576,438 read pairs
INPUT DNA
DNA per well     1.4 ng
Mean length +/- s.d. Â Â Â Â 103 kbp +/- 54 kbp
BARCODE (BC)
Reads with valid BC Â Â Â Â 94 %
Mean base quality score, BC bases     39
N50, read-pairs per input DNA molecule     3
Column
STRUCTURAL VARIANT CALLS
Large SVs     117
Deletions     36
PHASING
Phased SNPs     42 %
Genes phased in single phase block     28 %
Longest phase block     344 kb
N50 length, called phased blocks     61 kb
LINKED-READ ALIGNMENT
Long Ranger input reads     28,675,522
Mapped reads     0.97
Median insert size of aligned read pairs     303
Mean read depth     1.2
Reads marked as PCR duplicates     1.6 %
Non-N bases with zero coverage     41 %
Aligned bases mapped to target regions     NA %
Sequencing Quality
Column
Read 1 Q20
Read 1 Q30
Read 2 Q20
Read 2 Q30
Column
Sample Index Q20
Sample Index Q30
Barcode Q20
Barcode Q30
Barcodes
Row
Molecules
Row {.tabset .tabset-fade, data-height=200}
Size of the molecules
Number of reads per molecule
Column {.tabset .tabset-fade, data-height=200}
Number of reads per barcode
Number of molecules per barcode
